Hård, Joanna; Al Hakim, Ezeddin; Kindblom, Marie, et al. Conbase: a software for unsupervised discovery of clonal somatic mutations in single cells through read phasing. Genome Biology 20 (2019): 68. PubMed | PDF
Somatic mutations label cells that share a recent common ancestry and can be used to trace cell lineage in humans. Identification of somatic mutations is challenging due to errors and biases in single cell DNA sequencing data. Conbase enables the identification of somatic mutations with high confidence and low false discovery rate by taking advantage of read phasing.
Attenuation of the fibrotic scarring that is derived from a subpopulation of perivascular cells, termed type A pericytes, reduces fibrosis, astrogliosis and inflammation, and increases axon regeneration below the level of the lesion after spinal cord injury in mice. Mice with reduced fibrotic scarring showed improved functional recovery after injury. These findings identify pericyte-derived scarring as a target for therapies against spinal cord injury.
Stenudd, Moa; Sabelström, Hanna and Frisén, Jonas. Role of endogenous neural stem cells in spinal cord injury and repair. JAMA neurology 72.2 (2015): 235-237.
Magnusson, Jens P., et al. A latent neurogenic program in astrocytes regulated by Notch signaling in the mouse. Science 346.6206 (2014): 237-241.
Astrocytes in the striatum of adult mice activate a neurogenic program in response to experimental stroke or deletion of the Notch-mediating transcription factor Rbpj. They generate transit-amplifying cells that divide to generate ~40 neuroblasts per starting astrocyte. Some of the neuroblasts mature into neurons.
Spalding, Kirsty L.; Bergmann, Olaf, et al. Dynamics of hippocampal neurogenesis in adult humans. Cell 153.6 (2013): 1219-1227.
Carbon-14 dating of hippocampal neurons in humans showed ongoing neurogenesis in adulthood. A closer look at the dynamics of hippocampal neuron renewal revealed that one-third of all hippocampal neurons may be subject to exchange in an adult human. The annual turnover rate of hippocampal neurons within the group of renewing neurons is 1.75%.